2005年,瑞典Karolinska大学附属医院临床微生物系的研究人员建立了一种集宿主DNA消除、随机PCR扩增、高通量测序、生物信息学分析为一体的分子病毒学方法,以进行大规模的临床标本中新病毒的筛选[1]。该研究从下呼吸道感染患儿标本中检测到一种新的病毒,这一发现改变了30年来一 In 2005, researchers from the Department of Clinical Microbiology at the Karolinska University Hospital in Sweden established a molecular virology approach that integrates host DNA elimination, random PCR amplification, high-throughput sequencing and bioinformatics analysis for large-scale Screening of new viruses in clinical specimens [1]. The study detected a new virus from a sample of children with a lower respiratory tract infection, a finding that has changed over the past 30 years