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目的:考察南方红豆杉枝叶不同提取部位体外抑制α-葡萄糖苷活性和抗氧化活性。方法:对南方红豆杉的醇提液、石油醚部位、乙酸乙酯部位、正丁醇部位、水部位采用体外α-葡萄糖苷酶抑制模型进行酶抑制活性研究,并通过其清除1,1二苯基苦基苯肼(DPPH)自由基和Fe3±还原能力(FRAP)测定南方红豆杉提取物的体外抗氧化作用。结果:南方红豆杉提取物均有不同程度抑制α-葡萄糖苷酶活性,以乙酸乙酯部位效果最佳(IC5027.854 1 mg.L-1),其次醇提物(IC5029.215 5 mg.L-1)和正丁醇部位(IC5038.913 0 mg.L-1),水部位(IC5074.505 9 mg.L-1)石油醚部位(IC50128.729 5 mg.L-1)最弱。抗氧化活性依次为醇提液(DPPH IC506.370 7 mg.L-1,FRAP 4.266 mmol.g-1)>乙酸乙酯部位(DPPH IC509.535 8 mg.L-1,FRAP3.275 mmol.g-1)>正丁醇部位(DPPH IC5020.461 3 mg.L-1,FRAP 1.498 mmol.g-1)>水部位(DPPH IC5026.685 5 mg.L-1,FRAP 0.678 mmol.g-1)>石油醚部位。结论:初步确定南方红豆杉抑制α-葡萄糖苷酶活性有效成分主要在乙酸乙酯与正丁醇部位,抗氧化及其相关成分主要在乙酸乙酯部位。
OBJECTIVE: To investigate the inhibition of α-glucosidase activity and antioxidant activity of different extracts from the branches and leaves of Taxus chinensis. Methods: The inhibitory activity of alcohol extract, petroleum ether fraction, ethyl acetate fraction, n-butanol fraction and water fraction of Taxus chinensis var. Mairei was studied by in vitro inhibition of α-glucosidase. In Vitro Antioxidative Activity of Taxus chinensis Extracts Against Phenylphenyl Phenyl Phenylhydrazine (DPPH) and Fe3 ± Reducing Ability (FRAP). Results: All the extracts of Taxus cuspidata had different inhibitory effects on the activity of α-glucosidase, with the best effect of ethyl acetate (IC5027.854 1 mg.L-1) and the second alcohol extract (IC5029.215 5 mg. L-1) and n-butanol (IC5038.913 0 mg.L-1), and weakest in water (IC5074.505 9 mg.L-1) and petroleum ether (IC50128.729 5 mg.L-1). The antioxidant activity followed by alcohol extract (DPPH IC506.370 7 mg.L-1, FRAP 4.266 mmol.g-1)> ethyl acetate (DPPH IC509.535 8 mg.L-1, FRAP3.275 mmol. n-butanol fraction (DPPH IC5020.461 3 mg.L-1, FRAP 1.498 mmol.g-1)> water fraction (DPPH IC5026.685 5 mg.L-1, FRAP 0.678 mmol.g- 1)> Petroleum ether site. CONCLUSION: The active constituents inhibiting the α-glucosidase activity of Taxus chinensis mainly were ethyl acetate and n-butanol, and the antioxidant and related components were mainly in the ethyl acetate fraction.