论文部分内容阅读
目的:考察榭皮素(Quercetin)对S-亚硝基化谷胱甘肽(GSNO)诱导损伤的内皮细胞的保护作用,探讨其保护机制。方法:内皮细胞(EA.hy926)预先给予不同浓度榭皮素,之后给予GSNO损伤,建立模型。采用:噻唑兰染色(MTT法)检测细胞存活率测定,之后通过对超氧化物歧化酶(SOD),丙二醛(MDA),谷胱甘肽过氧化物酶(GSH-PX),活性氧(ROS),Caspase-3等指标测定进行分析。结果:榭皮素可明显提高内皮细胞内SOD,GSH-PX的活性,抑制MDA的产生,降低ROS的活性,减少细胞凋亡率,具有统计学差异(P<0.05)。结论:榭皮素对GSNO诱导损伤的细胞具有保护作用,其机制可能与榭皮素可提高细胞内抗氧化酶活性有关。
AIM: To investigate the protective effect of quercetin on endothelial cells induced by S-nitrosoglutated glutathione (GSNO) and its protective mechanism. Methods: Endothelial cells (EA.hy926) were pretreated with different concentrations of sebrenin and then given GSNO to establish model. The cell viability was determined by MTT assay. The effects of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-PX), reactive oxygen species (ROS), Caspase-3 and other indicators measured for analysis. Results: Sebiferexin significantly increased the activity of SOD and GSH-PX in endothelial cells, inhibited the production of MDA, decreased the activity of ROS and decreased the apoptosis rate (P <0.05). CONCLUSION: Sebrenol has a protective effect on GSNO-induced injury cells, and its mechanism may be related to the increase of intracellular antioxidant enzyme activity of sebrenin.