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目的:探讨脊髓损伤(SCI)后使用白藜芦醇(resveratrol, Res)对继发性脊髓水肿、受损脊髓组织中乳酸脱氢酶(LDH)与Na~+,K~+-ATP酶活性及早期脂质过氧化反应的影响。方法:采用重物下落撞击法制备成年大鼠的脊髓损伤模型,于损伤后即刻腹腔注射Res(50mg/kg,100mg/kg)或甲基强的松龙(MPSS,100mg/kg),观察给药后1h、24h、48h时Res组受损脊髓组织含水量、LDH与Na~+,K~+-ATP酶活性及MDA水平的变化,同时用电子显微镜观察超微结构的变化,并与MPSS组进行疗效对比。结果:Res显著抑制SCI后继发性水肿,48h最明显(P<0.05),抑制率为11.5％;显著降低LDH活性,24h时最大(P<0.01),抑制率大于40％;明显改善Na~+,K~+-ATP酶活性,以48h最显著(P<0.01),最大改善率在60％以上;显著抑制SCI后MDA产生,48小时为最明显(P<0.01),Res在50mg/kg和100mg/kg的剂量情况下所表现的上述作用比100mg/kg的MPSS更佳,电子显微镜的结果表明,Res和MPSS能有效保护SCI后脊髓组织的超微形态结构的正常性。结论:Res有效抑制脊髓损伤后的水肿和早期受损局部脂质过氧化反应并改善能量代谢系统,因此对脊髓损伤有潜在的治疗作用。
Objective: To investigate the activity of lactate dehydrogenase (LDH) and Na~+, K~+-ATPase in secondary spinal cord edema and injured spinal cord tissue after spinal cord injury (SCI) using resveratrol (Res). And the effects of early lipid peroxidation. METHODS: Spinal cord injury model was established in adult rats using weight drop impact method. Immediately after injury, Res (50 mg/kg, 100 mg/kg) or methylprednisolone (MPSS, 100 mg/kg) was intraperitoneally injected. At 1h, 24h and 48h after drug administration, changes in water content, LDH, Na+, K+-ATPase activity, and MDA levels in the injured spinal cord of Res group were observed. Changes in ultrastructure were observed by electron microscopy at the same time. The efficacy of the group was compared. Results: Res significantly inhibited the secondary edema after SCI, the most obvious 48h (P <0.05), the inhibition rate was 11.5%; LDH activity was significantly reduced, the maximum at 24h (P <0.01), the inhibition rate was greater than 40%; significantly improved Na ~ The activity of +, K~+-ATPase was the most significant at 48h (P<0.01), and the maximal improvement rate was above 60%; MDA production after SCI was significantly inhibited, the most obvious at 48 hours (P<0.01), Res at 50mg/ The above-mentioned effects of kg and 100 mg/kg were better than 100 mg/kg of MPSS. The results of electron microscopy showed that Res and MPSS can effectively protect the normality of ultrastructure of spinal cord after SCI. CONCLUSION: Res effectively inhibits edema and early impaired local lipid peroxidation after spinal cord injury and improves the energy metabolism system, and thus has a potential therapeutic effect on spinal cord injury.