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目的:研究姜黄素抗癌作用的分子机制。方法:以人膀胱癌细胞株EJ为模型,采用MTT法、流式细胞术,检测姜黄素对EJ细胞生长和凋亡的影响,并通过Western Blotting法检测姜黄素对EJ细胞PTEN/PI3K/AKT通路凋亡相关蛋白表达的影响。结果:姜黄素以浓度及时间依赖的方式抑制EJ细胞的增殖;流式细胞仪检测发现姜黄素作用于EJ细胞24 h后凋亡率呈剂量依赖性增加。Western Blotting显示50μM姜黄素作用EJ细胞后,PTEN、GSK-3β、C-raf、Bad、caspase-9、caspase-3的活性增强,PARP的裂解增加和Akt、PDK1的表达水平降低。结论:姜黄素能增加EJ细胞PTEN的表达,进而抑制PI3K/Akt信号通路的激活,继之激活下游GSK-3β,caspase-9和Bad等多种促凋亡分子的表达,诱导EJ细胞发生凋亡。该研究表明PTEN/PI3K/Akt信号通路在姜黄素诱导的EJ膀胱癌细胞凋亡中起了重要作用。
Objective: To study the molecular mechanism of curcumin’s anticancer effect. Methods: The human bladder cancer cell line EJ was used as a model. The effects of curcumin on the proliferation and apoptosis of EJ cells were detected by MTT assay and flow cytometry. The effects of curcumin on the expression of PTEN / PI3K / AKT Pathway apoptosis-related protein expression. Results: Curcumin inhibited the proliferation of EJ cells in a time-and concentration-dependent manner. Flow cytometry showed that the apoptotic rates of curcumin treated EJ cells in a dose-dependent manner increased in 24 h. Western Blotting showed that the activity of PTEN, GSK-3β, C-raf, Bad, caspase-9 and caspase-3 increased, the cleavage of PARP and the expression of Akt and PDK1 decreased after EJ cells were treated with 50μM curcumin. CONCLUSION: Curcumin can increase the expression of PTEN in EJ cells, and then inhibit the activation of PI3K / Akt signaling pathway, followed by the activation of downstream pro-apoptotic molecules such as GSK-3β, caspase-9 and Bad, and induce EJ cell apoptosis Death. The study shows that PTEN / PI3K / Akt signaling pathway plays an important role in curcumin-induced apoptosis in EJ bladder cancer cells.