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植物激素ABA参与不同的生理过程,尤其是在种子发育和非生物逆境的适应都需要ABA的调控。以水稻珍汕97和旱稻IRAT109为亲本的重组自交系群体为材料,分别调查种子发芽和苗期对ABA的敏感程度。种子发芽阶段以ABA处理下的相对发芽势(Relativegerminationvigor,RGV)和相对发芽率(Relativegerminationrate,RGR)为指标,苗期以ABA喷施处理下的卷叶程度(LeafrollingscoresbyABAspaying,LRS)和含ABA水培条件下的卷叶程度(LeafrollingscoresbyABAculturing,LRC)为指标。性状相关分析表明发芽阶段的相对发芽势与苗期卷叶程度呈显著正相关。用复合区间作图法和混合线性模型对ABA敏感性QTL定位和上位性效应分析。两种软件检测到的主效QTL位点大致相同。共检测到5个单位点QTL和6对上位性QTL与发芽阶段的ABA敏感性有关;8个单位点QTL和5对上位性QTL与水稻苗期对ABA的敏感性有关;在苗期,两种ABA处理条件下共检测到两个共同的QTL;仅一个共同的QTL同时控制发芽阶段和苗期对ABA的敏感性。这些研究结果说明,水稻对ABA的敏感性同时受单位点的多基因和上位性基因控制;而且控制种子萌发阶段发芽势和苗期对ABA敏感性的遗传基础有很大的不同。
Plant hormone ABA participates in different physiological processes, especially ABA regulation in seed development and abiotic stress adaptation. The recombinant inbred lines (Oryza sativa L.) Zhenshan97 and Upland rice IRAT10 were used as materials to investigate the sensitivity of ABA to seed germination and seedling stage respectively. Under the seed germination stage, relative germination potential (RGV) and relative germination rate (RGR) under ABA treatment were used as indexes. The leaf rolling degree under ABA spraying (LeafrollingscoresbyABAspaying, LRS) (LeafrollingscoresbyABAculturing, LRC) as an indicator. Correlation analysis of traits showed that the relative germination potential at the germination stage was significantly and positively correlated with the leaf volume at seedling stage. Mapping and Epistatic Effects of ABA Sensitive QTLs by Composite Interval Mapping and Mixed Linear Models. The major QTLs detected by either software were approximately the same. A total of five QTLs were detected and six pairs of epistatic QTLs were associated with ABA susceptibility at germination stage. Eight QTLs and five epistatic QTLs were associated with sensitivity to ABA in rice seedling stage. At the seedling stage, Two common QTLs were detected under ABA treatment. Only one common QTL controlled the sensitivity of ABA to germination and seedling stage. These results indicate that the sensitivity of rice to ABA is controlled by multiple loci and epistatic genes at the same time, and the genetic basis for controlling ABA susceptibility at the seed germination stage is very different.