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目的探讨镍污染区大气细颗粒物(PM2.5)对大鼠心血管系统损伤生物标志物的影响。方法于2007年6—7月和12月,采集镍污染区和对照区的大气PM2.5,进行部分元素分析;并将42只SPF级雄性Wistar大鼠随机分为生理盐水对照组、镍污染区及对照区PM2.5低(1.6mg/kg)、中(8.0mg/kg)和高(40mg/kg)剂量组,每组6只,经气管分别一次性滴注PM2.5悬液和生理盐水,染毒24h后处死大鼠,测定大鼠血清中可溶性血管细胞黏附分子(sVCAM-1)和心肌组织匀浆单核细胞趋化因子(MCP-1)的含量。结果镍污染区大气PM2.5中镍含量约是对照区的50倍。镍污染区组PM2.5染毒后大鼠心脏组织匀浆中MCP-1含量与染毒剂量成正相关,镍污染区各剂量组均显著性高于对照区各剂量组和生理盐水对照组(P﹤0.05)。镍污染区和对照区各剂量组染毒后大鼠血清中sVCAM-1含量均有所升高。镍污染区中、高剂量组与对照区相应剂量组比较,差异有统计学意义(P﹤0.05,P﹤0.01)。结论镍污染区大气PM2.5对大鼠血清sVCAM-1和心肌MCP-1影响较对照区大,其中镍可能起到了关键性作用。
Objective To investigate the effect of particulate matter (PM2.5) on the cardiovascular biomarkers in rats with nickel-contaminated area. Methods PM2.5 in nickel-contaminated and control areas was collected from June to July 2007 and December of 2007, and some elements were analyzed. Forty-two SPF male Wistar rats were randomly divided into physiological saline control group, nickel contamination PM2.5 low dose (1.6mg / kg), medium dose (8.0mg / kg) and high dose (40mg / kg) The rats were sacrificed 24h after exposure to saline. The contents of soluble vascular cell adhesion molecule (sVCAM-1) and monocyte chemoattractant monocyte chemotactic factor (MCP-1) in rat serum were measured. Results The nickel content of PM2.5 in the nickel-polluted area was about 50 times that of the control area. In the nickel-contaminated area, the content of MCP-1 in the heart tissue homogenate of the rats exposed to PM2.5 was positively correlated with the dose of nickel exposure. The nickel-contaminated area of each dose group was significantly higher than the control group and the saline control group P <0.05). The levels of sVCAM-1 in serum of rats in the nickel-contaminated area and the control area were increased. Compared with the corresponding dose group in the control group and the high dose group, the difference was statistically significant (P <0.05, P <0.01). Conclusions Atmospheric PM2.5 in nickel-contaminated area has a greater effect on serum sVCAM-1 and MCP-1 than control area, with nickel possibly playing a key role.