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背景与目的:食管癌早期可发生局部淋巴或血行转移,这是导致复发和预后差的主要原因。但是,食管癌转移发生的分子机制尚不清楚。本研究旨在分析食管癌原发灶和淋巴结转移灶肿瘤细胞染色体变化的特征,寻找或定位与食管癌转移相关基因,加深对其转移机制的了解。方法:应用比较基因组杂交技术(comparativegenomichybridization,CGH)分析15例食管癌患者原发灶和其对应的淋巴结转移灶的染色体基因组改变。结果:最常见染色体DNA拷贝数增加的部位是3q,8q,6p,20p,5p,18p,2p,2q,1q;常见的染色体DNA拷贝数丢失的部位是10p,10q,17p,18q,4p,13q。其中,最有意义的发现是6p增加(原发灶:2/15,13%,转移灶:7/15,47%),20p增加(原发灶:5/15,33.3%,转移灶:11/15,73.3%)。第二个发现是10p丢失(原发灶:2/15,13.3%,转移灶:8/15,53%),10q丢失(原发灶:2/15,13.3%,转移灶:7/15,46.6%)。结论:食管癌原发灶和淋巴结转移灶细胞染色体基因组改变最显著的部位是6p,20p的增加和10p,10q的丢失;这些部位可能存在与食管癌细胞淋巴结转移相关的基因。
BACKGROUND & OBJECTIVE: Local lymphoid or hematogenous metastasis may occur early in esophageal cancer, which is the main reason leading to relapse and poor prognosis. However, the molecular mechanism of esophageal cancer metastasis is unclear. The purpose of this study was to analyze the characteristics of chromosomal changes in primary esophageal cancer and lymph node metastasis tumor cells, to find out or locate the genes associated with esophageal cancer metastasis, and to deepen the understanding of its metastasis mechanism. Methods: The genomic changes of primary esophageal cancer and its corresponding lymph node metastases were analyzed by comparative genomic hybridization (CGH). Results: The most common chromosomal DNA copies were 3q, 8q, 6p, 20p, 5p, 18p, 2p, 2q and 1q. The common sites of chromosome DNA loss were 10p, 10q, 17p, 18q, 4p, 13q. Among them, the most significant finding was the increase of 6p (primary tumor: 2 / 15,13%, metastasis: 7/15, 47%), 20p increased (primary tumor: 5/15, 11/15, 73.3%). The second finding was the loss of 10p (primary lesions: 2/15, 13.3%, metastases: 8/15, 53%), 10q loss (primary lesions: 2/15, 13.3%, metastases: 7/15 , 46.6%). CONCLUSION: The most significant chromosomal location of esophageal cancer and lymph node metastasis is the increase of 6p, 20p and the loss of 10p and 10q. These genes may be involved in lymph node metastasis of esophageal cancer cells.