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目的:测定胸腺肽溶液中α_1的含量。方法:以胸腺肽α_1纯品作为标准品,用高效毛细管电泳测定,未涂层石英毛细管柱(78cm×30μm);电泳缓冲溶液为0.05mol/L磷酸盐缓冲液(pH7.0);运行电压15kv;负压进样(66.7kPa);温度25℃;检测波长200nm:进样时间1秒。用比例色谱的方法确定胸腺肽溶液中α_1峰的纯度。结果:胸腺肽α_1的标准溶液的线性范围50～800μg/ml,低、中、高三种浓度的平均回收率分别为87％、91％、97％。分离出的胸腺肽溶液中α_1的组份峰为单一组份峰,三批胸腺肽溶液中α_1的质量百分比分别为1.24％,0.812％,0.732％。结论:该方法可用于胸腺肽质控中α_1含量的测定。
Objective: To determine the content of α_1 in thymosin solution. Methods: Thymosin α 1 1 was used as a standard sample and determined by high performance capillary electrophoresis. Uncoated quartz capillary column (78 cm × 30 μm) was used. The electrophoresis buffer was 0.05 mol / L phosphate buffer (pH 7.0). The operating voltage was 15 kv ; Negative pressure injection (66.7kPa); Temperature 25 ℃; Detection wavelength 200nm: Injection time 1 second. The purity of α_1 peak in thymosin solution was determined by the method of ratio chromatography. Results: The linear range of thymosin α_1 standard solution was 50 ~ 800μg / ml. The average recoveries of low, middle and high concentrations were 87%, 91% and 97% respectively. The fraction of α_1 isolated from the thymosin solution was a single component peak. The mass percent of α_1 in three batches of thymosin solution was 1.24%, 0.812% and 0.732%, respectively. Conclusion: This method can be used to determine the content of α_1 in thymosin control.