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【目的】2株炭疽芽胞杆菌(Bacillus anthracis)17003-14和17003-32的多位点序列分型(Multilocussequence typing,MLST)研究。【方法】选取B.anthracis基因组7个常见管家基因位点glpF、gmk、ilvD、pta、pur、pycA和tpi进行PCR扩增、测序,与MLST数据库中的等位基因序列进行比对,确定菌株的序列型(sequence type,ST)。【结果】B.anthracis 17003-14和17003-32的等位基因编号分别为113、31、1、43、1、53、7和113、31、1、43、1、53、37,比对结果显示这2株细菌的等位基因编号组合未见报道。【结论】17003-14和17003-32为新ST菌株,已被MLST数据库确认,注册号(pubMLST id)分别为id-1053和id-1054。
【Objective】 Multilocus sequence typing (MLST) studies of two strains of Bacillus anthracis 17003-14 and 17003-32 were performed. 【Method】 The sequences of glpF, gmk, ilvD, pta, pur, pycA and tpi in seven common housekeeping gene loci of B. anthracis were selected for PCR amplification, sequencing and comparison with the allele sequences in the MLST database to identify the strains The sequence type (ST). 【Result】 The allele numbers of B. anthracis 17003-14 and 17003-32 were 113,31,1,43,1,53,7 and 113,31,1,43,1,53,37, respectively The results showed that these two strains of alleles number combination has not been reported. 【Conclusion】 17003-14 and 17003-32 are new ST strains, which have been confirmed by MLST database. The pubMLST id are id-1053 and id-1054, respectively.