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目的探讨急慢性乙型肝炎患者外周血Ｔ细胞亚群及可溶性ＩＬ＿２Ｒ水平的变化，为诊断病情演变和预后判定寻找可靠的依据．方法应用ＡＰＡＡＰ桥联酶标法和双抗体夹心ＥＬＩＳＡ法检测正常人３０例和各型乙型肝炎患者１７５例的Ｔ细胞亚群变化和血清可溶性ＩＬ＿２Ｒ（ｓＩＬ＿２Ｒ）水平．结果各组乙肝患者ＣＤ３，ＣＤ４，ＣＤ４／ＣＤ８比值明显降低，ＣＤ８则明显升高．乙肝患者血清ｓＩＬ＿２Ｒ水平均显著升高，其中慢重肝、肝硬变、急性乙肝和慢性肝炎增高最为显著，与正常对照组相比Ｐ均＜００１，且与ＨＢＶ复制有关．结论乙型肝炎患者存在Ｔ细胞比例失衡，血清ｓＩＬ＿２Ｒ水平均明显增高，其水平与机体免疫功能状态及肝细胞损伤程度相关．
Objective To investigate the changes of T lymphocyte subsets and soluble IL - 2R in peripheral blood of patients with acute and chronic hepatitis B, and to find a reliable basis for the diagnosis of disease progression and prognosis. Methods The changes of T cell subsets and serum soluble IL 2 R (sIL 2 R) levels in 30 normal subjects and 175 hepatitis B patients were detected by APAAP double-labeled enzyme-linked immunosorbent assay (ELISA) and double antibody sandwich ELISA. Results The ratio of CD3, CD4 and CD4 / CD8 in patients with hepatitis B in each group was significantly decreased, and CD8 was significantly increased. Serum sIL_2R levels were significantly elevated in patients with hepatitis B, of which chronic severe hepatitis, cirrhosis, acute hepatitis B and chronic hepatitis increased most significantly, P <0.01 compared with the normal control group, and HBV replication. Conclusion The imbalance of T cells and the level of serum sIL_2R in patients with hepatitis B were significantly higher than those in controls. The levels of sIL_2R in serum were significantly correlated with immune status and hepatocellular injury.