为建立一种简易的检测ＨＢＶ前Ｃ区Ａ８３点突变的方法，设计了一种针对Ａ８３变异株的错配引物，通过ＰＣＲ，可在Ａ８３变异株的扩增产物中引入一个ＢＳＵ３６Ｉ限制酶位点，将扩增产物（１０２ｂｐ）用该酶消化，含变异者显示８２ｂｐ的酶切片段，以此判定的变异与测序结果一致。用本法检测了慢性乙型肝炎１２例和随访ＨＢｅ血清转换者５例，结果表明前Ｃ区Ａ８３变异与病变发展一致。 In order to establish a simple method to detect A83 point mutation in pre-HBV C region, a mismatch primer designed for A83 mutant was designed. By PCR, a BSU36I restriction enzyme site was introduced into the amplified product of A83 mutant The amplified product (102bp) was digested with this enzyme. The mutants showed a 82bp digested fragment, which was consistent with the sequencing results. Using this method to detect 12 cases of chronic hepatitis B and follow-up HBe seroconversion in 5 cases, the results show that the pre-C area A83 mutation consistent with the development of lesions.