论文部分内容阅读
目的:检测前列腺癌组织中GSTP1和DAPK基因异常甲基化,探讨其甲基化与前列腺癌临床病理特征间的关系。方法:采用巢式甲基化特异性PCR(nested methylation specific polymerase chain reaction,NMSP)法对57例前列腺癌组织和35例良性前列腺增生组织进行甲基化检测,分析其甲基化的发生与前列腺癌临床病理特征间的关系。结果:前列腺癌组织中GSTP1和DAPK基因甲基化检出率显著高于良性前列腺增生组织(GSTP1:61.4%vs 2.9%;DAPK:43.9%vs 8.6%;均P<0.01)。GSTP1基因甲基化率在不同Gleason评分间具有明显差异(χ2=11.53,P=0.001),而在不同年龄、前列腺特异性抗原(PSA)和临床分期之间差异却无显著性(χ2=0.111,1.662和1.975,均P>0.05);DAPK基因甲基化率在不同年龄、PSA、Gleason评分和不同临床分期之间均无明显差异(χ2=0.071、0.002、1.290和2.309,P均>0.05)。结论:GSTP1和DAPK基因异常甲基化与前列腺癌的发生与发展相关,可有助于前列腺癌的诊断。
Objective: To detect abnormal methylation of GSTP1 and DAPK genes in prostate cancer and to explore the relationship between methylation and clinicopathological features of prostate cancer. Methods: The methylation of 57 cases of prostate cancer and 35 cases of benign prostatic hyperplasia was detected by nested methylation specific polymerase chain reaction (NMSP) Relationship between clinicopathological characteristics of cancer. Results: The methylation rates of GSTP1 and DAPK genes in prostate cancer tissues were significantly higher than those in benign prostatic hyperplasia (GSTP1: 61.4% vs 2.9%; DAPK: 43.9% vs 8.6%; all P <0.01). The methylation rate of GSTP1 gene was significantly different between different Gleason scores (χ2 = 11.53, P = 0.001), but no significant difference was found between different age, PSA and clinical stage (χ2 = 0.111 , 1.662 and 1.975, all P> 0.05). There was no significant difference in the methylation rates of DAPK gene among different ages, PSA, Gleason score and clinical stage (χ2 = 0.071,0.002,1.290 and 2.309, P> 0.05 ). Conclusion: The abnormal methylation of GSTP1 and DAPK genes is associated with the occurrence and development of prostate cancer, which may contribute to the diagnosis of prostate cancer.