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目的研究端粒酶逆转录酶(TERT)和细胞增殖核抗原(PCNA)在喉鳞癌组织及细胞中的表达及其临床病理学意义。方法采用连续光谱扫描量子点免疫荧光组织化学方法检测喉鳞癌组织芯片中TERT和PCNA蛋白的表达情况;靶向抑制喉癌细胞株Hep-2的TERT,运用RT-PCR检测TERT和PCNA mRNA的表达情况。结果良恶性头颈部肿瘤中TERT和PCNA的表达差异有统计学意义(P<0.01)。TERT在病理分级III级组中的阳性率明显高于Ⅰ~Ⅱ级组(P<0.05),PCNA表达在病理分级Ⅲ级组中的阳性率显著高于Ⅰ~Ⅱ级组(P<0.01),且TERT和PCNA的表达呈中度正相关性(r=0.577,P<0.05);细胞实验中,靶向抑制TERT后,喉癌细胞株Hep-2增殖能力明显下降,TERT mRNA表达下调80%,PCNA mRNA表达同时下调70%。结论 TERT和PCNA与喉鳞癌的发生相关,且两者有协同作用,TERT影响肿瘤细胞增殖能力可能与PCNA表达变化有关。
Objective To investigate the expression of telomerase reverse transcriptase (TERT) and proliferating cell nuclear antigen (PCNA) in laryngeal squamous cell carcinoma and their clinicopathological significance. Methods The expression of TERT and PCNA protein in laryngeal squamous cell carcinoma (LSCC) was detected by using continuous scanning electron microscopy and immunofluorescence histochemistry. Targeted inhibition of TERT in laryngeal carcinoma cell line Hep-2 was detected by RT-PCR. Express the situation. Results The expression of TERT and PCNA in benign and malignant head and neck tumors had statistical significance (P <0.01). The positive rate of TERT in pathological grade III group was significantly higher than that in grade Ⅰ ~ Ⅱ group (P <0.05). The positive rate of PCNA expression in pathological grade Ⅲ group was significantly higher than that in grade Ⅰ ~ Ⅱ group (P <0.01) (R = 0.577, P <0.05). In the experiment of cell-targeted inhibition of TERT, the proliferation of Hep-2 cell line was significantly decreased and the expression of TERT mRNA was down-regulated by 80 %, PCNA mRNA expression down 70% at the same time. Conclusion TERT and PCNA are correlated with the occurrence of laryngeal squamous cell carcinoma, and both of them have synergistic effect. TERT may affect the proliferation of tumor cells possibly related to the change of PCNA expression.