论文部分内容阅读
目的:研究尼古丁对败血症大鼠晚期炎症介质HMGB-1血清浓度及存活率的影响。方法:盲肠结扎穿孔法建立败血症大鼠模型,应用随机数字表随机分为5组(假手术组,空白对照组,生理盐水组,阳性对照组,尼古丁组)。运用酶联免疫吸附试验检测各组大鼠血清中TNF-α、IL-6及HMGB-1等炎症因子,比较浓度变化情况。Kaplan-Meier法对各组大鼠存活率经行分析,比较不同剂量尼古丁,不同时间点给药对败血症大鼠存活率的影响。结果:各组大鼠血清TNF-α、IL-6浓度在模型建立后即开始增高,空白对照及生理盐水组较其它3组增高明显(p<0.05),在建模后第2天达最高值后开始下降。各组HMGB-1浓度在模型建立后第2天开始显著增高,尼古丁组及假手术组较其它3组增加缓慢(p<0.05),到第5天达最高值后均开始下降。尼古丁的最佳作用浓度为200ug/kg体重,最佳给药时间为模型建立后24h。与其它四组相比,尼古丁能显著降低败血症大鼠的血清HMGB-1浓度(p<0.05),对败血症大鼠存活率的影响,尼古丁组与生理盐水组及空白对照组相比差异有统计学意义(p<0.05),与假手术组及阳性对照组相比差异无统计学意义(p>0.05)。结论:尼古丁能显著降低败血症大鼠血清HMGB-1浓度,提高败血症大鼠的存活率,对败血症大鼠具有一定的治疗作用。
Objective: To study the effects of nicotine on the serum concentration of HMGB-1 and the survival rate of late inflammatory mediators in septic rats. Methods: The model of sepsis was established by cecal ligation and perforation method and randomly divided into 5 groups (sham operation group, blank control group, normal saline group, positive control group and nicotine group) by random number table. Serum TNF-α, IL-6 and HMGB-1 and other inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA). The Kaplan-Meier method was used to analyze the survival rate of rats in each group. The effects of different doses of nicotine and different time points on the survival rate of septic rats were compared. Results: Serum levels of TNF-α and IL-6 in each group began to increase at the beginning of the model establishment, and were significantly increased in the blank control group and saline group compared with the other three groups (p <0.05), and reached the highest on the second day after modeling Value began to decline. The concentration of HMGB-1 in each group began to increase significantly from the second day after the model was established. The nicotine group and the sham operation group increased slowly (p <0.05) compared with the other three groups, and began to decline after reaching the highest value on the fifth day. The optimal concentration of nicotine was 200ug / kg body weight, the best time for administration was 24h after model establishment. Compared with the other four groups, nicotine can significantly reduce the serum concentration of HMGB-1 in sepsis rats (p <0.05), the survival rate of septic rats, nicotine group compared with the saline group and the blank control group statistics (P <0.05). There was no significant difference between the sham group and the positive control group (p> 0.05). CONCLUSION: Nicotine can significantly reduce the serum concentration of HMGB-1 in septic rats, increase the survival rate of septic rats, and have certain therapeutic effect on sepsis rats.