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High performance liquid chromatography (HPLC) was used to determine the degradation efficiency of bacteria 2 strain (B2S) under different conditions, the optimum cultivation conditions for fomesafen degradation bacterium B2S were as the followings:temperature 35℃; inoculation quantity 5%; pH 5.0; glucose content 0.5% and fomesafen concentration 10 mg ? L-1. Under optimal conditions, B2S degraded fomesafen within 72 h of fomesafen application, with a degradation rate of nearly 100%. High performance liquid chromatography-mass spectrometry (HPLC-MS) was used to analyze fomesafen degradation into microbial products. A more thorough understanding of microbial fomesafen degradation mechanisms was discussed. The pathway of fomesafen degradation by B2S was also inferred herein.