支原体为临床致儿童肺炎的一种重要的病原体,本病过去长期借助于血清抗体滴度测定及病原体分离培养等。但因存在耗时较久,检出阳性率低以及灵敏性和特异性欠佳等缺点,不能达到为临床提供早期准确诊断的目的。采用核酸分子杂交技术与传统聚合酶链反应(PCR)二者相结合的检测方法, Mycoplasma pneumonia caused by clinical children is an important pathogen, the past long-term use of serum antibody titer and pathogen isolation and culture. However, due to the existence of time-consuming, low positive rate and the sensitivity and specificity of the shortcomings, can not achieve the purpose of providing early accurate diagnosis of clinical purposes. Using nucleic acid hybridization technology and traditional polymerase chain reaction (PCR) combination of both detection methods,