论文部分内容阅读
利用溴和萤光素的卤代反应测定微量溴早有报导。青海盐湖研究所的资料认为在pH5.5醋酸～醋酸钠缓冲溶液中显色后,再加入氢氧化钠增大pH至5.8,可以使红色稳定5分钟。此条件不够理想。我们改为盐酸～醋酸铵缓冲溶液并确定于pH3.8～4.2显色,有良好重现性。显色后加入甲酸钠并加热至60℃～70℃,可破坏残留氧化剂而使红色长期稳定。溴代萤光素能够被正丁醇完全萃取,籍此可以提高分析灵敏度。天然水和盐矿中共存离子均不干扰,氯离子可以加速反应,含量在60～120毫克效果最好。本法适合分析
The use of bromine and luciferin halogenation reaction of trace bromine has been reported. According to the data of Qinghai Salt Lake Research Institute, after color development in pH5.5 acetate-sodium acetate buffer solution, adding sodium hydroxide to increase the pH to 5.8, the red color can be stabilized for 5 minutes. This condition is not ideal. We changed to hydrochloric acid ~ ammonium acetate buffer solution and determined at pH 3.8 ~ 4.2 color, with good reproducibility. Color after adding sodium formate and heating to 60 ℃ ~ 70 ℃, can destroy the residual oxidant and make the red long-term stability. Bromo-Luciferin can be fully extracted with n-butanol, which enhances the sensitivity of the assay. Natural water and salt coexist ions are not interfering, chloride ions can speed up the reaction, the content of 60 to 120 mg is the best. This law is suitable for analysis