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目的以硝酸钙作为基体改进剂,建立血中锡的石墨炉原子吸收光谱法。方法以硝酸钙作为基体改进剂对石墨管进行预处理,抗凝全血用曲拉通溶液稀释后直接进样,由石墨炉原子吸收光谱法进行检测;研究并对比了几种常见基体改进剂、基体改进剂浓度及用量、仪器条件等对测定血锡的影响,以确定最佳的实验条件。结果硝酸钙可以提高血锡测定的灵敏度,降低背景干扰,是较为理想的基体改进剂。血锡含量在8.0μg/L~40.0μg/L时,线性关系良好,相关系数(r)为0.999 5,最低检出限为1.29μg/L。相对标准偏差(RSD)为1.64%~6.39%。加标回收率为90.81%~101.08%。样品在4℃冰箱内可保存14 d。Mn、Pb、Zn不干扰本法对血锡的测定。结论研制的测定方法具有快速、灵敏、准确、精密度高、干扰少等优点,适用于血液中锡的测定。
Objective To establish a graphite furnace atomic absorption spectrometry method for the determination of tin in blood with calcium nitrate as matrix modifier. Methods Calcium nitrate was used as a matrix modifier to pretreat the graphite tube. The anticoagulant whole blood was directly diluted with Triton solution and then detected by graphite furnace atomic absorption spectrometry. Several common matrix modifiers , Matrix improver concentration and dosage, instrument conditions, etc., to determine the effect of blood tin to determine the best experimental conditions. Results Calcium nitrate can improve the sensitivity of determination of blood tin, reduce background interference, is an ideal matrix modifier. The linearity was good with the content of tin in the range of 8.0μg / L to 40.0μg / L with the correlation coefficient (r) of 0.999 5 and the minimum detectable limit of 1.29μg / L. The relative standard deviations (RSDs) ranged from 1.64% to 6.39%. Spike recovery was 90.81% ~ 101.08%. Samples can be stored in a refrigerator at 4 ℃ for 14 days. Mn, Pb, Zn do not interfere with the determination of blood tin. Conclusion The developed method has the advantages of fast, sensitive, accurate, high precision and less interference, and is suitable for the determination of tin in the blood.