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[目的]探讨谷胱甘肽转硫酶基因多态性M1(GSTM1)、T1(GSTT1)及烟酒嗜好与胃癌易感性的关系,并对GST基因多态性GSTT1、GSTM1与烟酒暴露在胃癌发生中的交互作用进行分析。[方法]采用1:l配对病例-对照研究方法和PCR技术,检测121例原发性胃癌患者和相应对照的GSTM1和GSTT1基因型,结合研究对象的烟酒嗜好,应用SAS统计分析系统,分析GSTM1和GSTT1基因型及烟酒暴露与胃癌发病的关系,并对基因-基因,基因-烟酒暴露在胃癌发生中的交互作用进行分析。[结果]GSTM1(-)基因型频率在病例组和对照组中分别占44.63%和33.88%,分布无显著性差异(x2=2.6436,P>0.05),GSTT1(-)基因型频率在病例组和对照组中分别为52.89%和44.63%,分布也无显著性差异(x2=1.1650,P>0.05)。吸烟者比非吸烟者发生胃癌的危险高(OR=2.538,95%CI:1.336-4.823);饮酒者比非饮酒者发生胃癌的危险高(OR=2.097,95%CI:1.025-4.291)。同时携带GSTM1(-) 和GSTT1(-)基因型者发生胃癌的危险性高于GSTM1(+)和GSTT1(+)基因型携带者(OR=2.097,95%CI:1.025-4.291);同时有烟酒嗜好的个体发生胃癌的危险性高于无烟酒嗜好者(OR=2.330,95%CI:1.211-4.482)。携带GSTM1(-)和GSTT1(-) 且有烟酒嗜好者,发生胃癌的危险显著高于携带GSTMI(+)和GSTT1(+)的无烟酒嗜好者(OR=3.600,95%CU:1.025-12.650)。[结论]吸烟、饮酒与胃癌易感性增加有关,GSTM1和GSTT1 基因型及烟酒嗜好在胃癌发生中存在一定的交互作用。
[Objective] To investigate the relationship between GSTM1, T1 (GSTT1), smoking and drinking preferences and the susceptibility to gastric cancer, and to investigate the relationship between GST polymorphism GSTT1, GSTM1 and smoking and drinking Interactions in gastric carcinogenesis were analyzed. [Method] The genotypes of GSTM1 and GSTT1 in 121 patients with primary gastric cancer and their corresponding controls were detected by 1: 1 matched case-control study and PCR technique. Combined with the alcohol and tobacco preferences of subjects, SAS statistical analysis system was used to analyze GSTM1 and GSTT1 genotypes and the relationship between tobacco and alcohol exposure and the incidence of gastric cancer, and the gene-gene, gene-tobacco alcohol and tobacco exposure in the occurrence of gastric cancer in the interaction analysis. [Results] The frequency of GSTM1 (-) genotype was 44.63% and 33.88% respectively in the case group and the control group, with no significant difference (x2 = 2.6436, P> 0.05). The frequency of GSTT1 (- And the control group were 52.89% and 44.63%, respectively, with no significant difference (x2 = 1.1650, P> 0.05). Smokers had a higher risk of developing gastric cancer than non-smokers (OR = 2.538, 95% CI: 1.336-4.823); drinkers had a higher risk of developing gastric cancer than non-drinkers (OR = 2.097, 95% CI: 1.025-4.291). Patients with both GSTM1 (-) and GSTT1 (-) genotypes had a higher risk of developing gastric cancer than those with GSTM1 (+) and GSTT1 (+) genotypes (OR = 2.097, 95% CI: 1.025-4.291) Individuals who smoke and drink alcohol are at a higher risk of gastric cancer than those who smoke and smoke (OR = 2.330, 95% CI: 1.211-4.482). The risk of developing gastric cancer was significantly higher in GSTM1 (-) and GSTT1 (-) patients with alcohol and alcoholism than those with GSTMI (+) and GSTT1 (+) (OR = 3.600, 95% CU: 1.025 -12.650). [Conclusion] Smoking and alcohol consumption are related to the increased susceptibility to gastric cancer. The genotypes of GSTM1 and GSTT1 and alcohol and tobacco preferences have some interactions in the development of gastric cancer.