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目的:研究急性白血病(AL)患者MK和VEGF基因的表达水平及其与血管新生、临床预后的关系。方法:应用实时荧光定量PCR检测95例不同病程的AL患者骨髓单个核细胞MK和VEGF mRNA的表达。结果:急性非淋巴细胞白血病(AML)和急性淋巴细胞白血病(ALL)患者MK和VEGF表达量均明显高于对照组,P=0.000 0。初治/复发组AL患者MK、VEGF基因表达水平较对照组及完全缓解组升高,完全缓解组MK和VEGF表达水平仍较对照组高,P均<0.01。缓解组MK表达水平与VEGF和骨髓原幼细胞数存在显著相关性,r=0.673,P<0.01;r=0.468,P<0.01。复发组MK和VEGF之间也存在相关性,r=0.526,P<0.05。初治组和对照组MK、VEGF、骨髓原幼细胞数之间无明显相关性。结论:MK、VEGF高表达可能与AL血管新生、病情进展有关,且两者存在一定的协同作用,共同监测可能对研究急性白血病的发病及指导治疗和估计预后有一定意义。
Objective: To study the expression of MK and VEGF gene in patients with acute leukemia (AL) and its relationship with angiogenesis and clinical prognosis. Methods: The expression of MK and VEGF mRNA in bone marrow mononuclear cells from 95 AL patients with different course of disease was detected by real-time fluorescence quantitative PCR. Results: The expressions of MK and VEGF in acute non-lymphocytic leukemia (AML) and acute lymphoblastic leukemia (ALL) were significantly higher than those in control group (P = 0.000 0). The MK and VEGF gene expression levels in newly diagnosed and relapsed AL patients were higher than those in control group and complete remission group. The MK and VEGF expression in complete remission group were still higher than those in control group (all P <0.01). There was a significant correlation between MK expression levels and the number of primitive myeloid progenitor cells in remission group, r = 0.673, P <0.01; r = 0.468, P <0.01. There was also a correlation between MK and VEGF in relapse group, r = 0.526, P <0.05. The initial treatment group and the control group MK, VEGF, no significant correlation between the number of primitive bone marrow cells. Conclusion: The overexpression of MK and VEGF may be related to AL angiogenesis and progression of the disease, and there is a synergistic effect between the two. Co-monitoring may be of significance in the study of the pathogenesis, treatment and prognosis of acute leukemia.