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目的 观察木黄酮 (genistein,GST)对培养的人垂体腺瘤细胞增殖的影响 .方法 将 GST作用于体外培养的人垂体腺瘤细胞 ,测定 MTT值及 3H- Td R参入量 ,并用流式细胞仪测定细胞周期 .结果 不同浓度的 GST(10 - 7,10 - 6 和10 - 5 mol· L- 1 )明显抑制垂体腺瘤细胞的增殖 ,MTT的A490 nm 值变化率分别为 (91.2± 4.9) % ,(78.8± 5 .3) %和(6 5 .8± 5 .6 ) % ,(P<0 .0 1) ,cpm值的变化率分别为 (88.6±3.6 ) % ,(75 .5± 5 .7) %和 (5 8.9± 4.9) % ,(P<0 .0 1) ,并存在着剂量效应 . 10 - 5 mol· L- 1 的 GST可使 G1期细胞比例从对照组的 6 8.2 %上升为 91.0 % (P<0 .0 1) .木黄酮对垂体腺瘤细胞的作用与雌激素受体无关 .结论 GST在体外能明显抑制培养的人垂体腺瘤细胞的增殖
Objective To observe the effect of genistein (GST) on the proliferation of cultured human pituitary adenoma cells. Methods GST was applied to human pituitary adenoma cells cultured in vitro to determine MTT value and 3H-Td R incorporation, and flow cytometry was used. The cell cycle was measured. Results Different concentrations of GST (10 -7,10 -6 and 10 -5 mol·L - 1 ) significantly inhibited the proliferation of pituitary adenoma cells. The rate of change of MTT A490 nm was (91.2 ± 4.9). %, (78.8 ± 5.3)% and (65.8 ± 5.6)%, (P <0.01), and the rate of change of cpm was (88.6 ± 3.6)%, (75). 5 ± 5 .7) % and (5 8.9 ± 4.9) %, (P <0.01), and there is a dose effect. GST of 10 - 5 mol·L - 1 can increase the proportion of G1 cells from the control group. The 68.2% increase was 91.0 % (P<0.01). The effect of lignan flavone on pituitary adenoma cells was not related to estrogen receptor. Conclusions GST can significantly inhibit the proliferation of cultured human pituitary adenoma cells in vitro.