论文部分内容阅读
观察超抗原SEA(D227A)的真核表达载体(pmSEA),对HBVDNA疫苗诱导Balbc小鼠(H2d)免疫应答的调节作用。肌内注射空载体pcDNA3、HBVDNA疫苗加pmSEA佐剂(pHBVS2S+pmSEA)或不加佐剂(pHBVS2S);ELISA法测定血清抗HBs;ELISPOT检测分泌IFNγ的脾淋巴细胞;4h51Cr释放法检测小鼠脾细胞CTL活性。HBVDNA佐剂组免疫小鼠抗HBsAg抗体滴度明显高于不加佐剂组,其IgG1IgG2a的比例不同于多肽免疫组,二者分别为0.282与10。HBVDNA佐剂组均能增强IgG1和IgG2a的产生,是不加佐剂组的1.36、1.73倍。佐剂组小鼠脾淋巴细胞IFNγ的分泌量是不加佐剂组2～3倍。CTL细胞杀伤活性(E:T=100)佐剂组与不加佐剂组分别为:69.77%±7.5%、42.81%±7.7%,差异显著(P<0.05)。HBVDNA疫苗具有较强的免疫原性,能够诱导机体产生特异性的抗体及CTL反应;pmSEA佐剂能够提高小鼠对DNA疫苗的免疫应答,有望成为DNA疫苗的免疫佐剂。
The eukaryotic expression vector (pmSEA) of superantigen SEA (D227A) was observed to regulate the immune response induced by HBVDNA vaccine in Balbc mice (H2d). The empty vector pcDNA3, HBVDNA vaccine plus pmSEA adjuvant (pHBVS2S + pmSEA) or without adjuvant (pHBVS2S); ELISA anti-HBs; ELISPOT spleen lymphocytes secreting IFNγ; 4h51Cr release assay mouse spleen cells CTL activity. The anti-HBsAg antibody titer of HBVDNA-immunized mice was significantly higher than that of the non-adjuvanted mice, and the ratio of IgG1 IgG2a was different from that of the peptide immunized mice, which were 0.282 and 10 respectively. HBVDNA adjuvant group can enhance the production of IgG1 and IgG2a, 1.36,1.73 times that of the adjuvant-free group. Adjuvant group of mice splenic lymphocytes IFNγ secretion was not added adjuvant group 2 to 3 times. The cytotoxicity of CTL (E: T = 100) were 69.77% ± 7.5% and 42.81% ± 7.7%, respectively, with significant difference (P <0.05). HBVDNA vaccine has strong immunogenicity, can induce the body to produce specific antibodies and CTL response; pmSEA adjuvant can improve the immune response to DNA vaccine in mice, is expected to become DNA vaccine immunoadjuvant.