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扩展蛋白是植物细胞壁延伸过程中的关键调节因子,在植物的生长发育以及对逆境的响应过程中起着重要作用。本文选用小麦(HF 9703)胚芽鞘为材料,采用Hepes法和SDS法分别提取小麦胚芽鞘扩展蛋白,通过改良的植物组织伸长测定仪测定其活性,并利用扩展蛋白抗体进行免疫印迹以检测其丰度,主要研究了小麦胚芽鞘扩展蛋白的特性及对水分胁迫的响应。结果表明:Hepes法提取的扩展蛋白活性较高,而SDS法的提取效率高;离体小麦胚芽鞘扩展蛋白的活性具有pH依赖性,且随缓冲液的交替更换(pH 4.5:pH 6.8)而反复逆转;扩展蛋白主要定位于细胞壁中;小麦胚芽鞘扩展蛋白和黄瓜下胚轴扩展蛋白具有交叉重组活性,但这种活性具有种属特异性。水分胁迫诱导小麦胚芽鞘扩展蛋白的活性和丰度提高,扩展蛋白活性的提高在小麦对水分胁迫的抗性方面可能具有重要作用。
Extensin is a key regulator of plant cell wall elongation and plays an important role in the growth and development of plants and their response to stress. In this paper, the coleoptile of wheat (HF 9703) was used as material, Hepes and SDS methods were used to extract the expanded proteins of wheat coleoptile, the activity was measured by a modified plant tissue elongation tester, and the antibody was used to detect the protein Abundance, the main characteristics of wheat coleoptile expand protein and its response to water stress. The results showed that the Hepes extraction of the extended protein activity is high, but the extraction efficiency of SDS method is high; the activity of ex-vivo wheat coleoptile expand protein is pH-dependent, and with alternating buffer (pH 4.5: pH 6.8) and Repeatedly reversed; expansion of protein located in the cell wall; wheat coleoptile expand protein and cucumber hypocotyls extended protein cross-reorganization activity, but this activity is species-specific. Under water stress, the activity and abundance of expanded proteins of wheat coleoptile increased, and the increase of expand protein activity may play an important role in the resistance of wheat to water stress.