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制备兔抗小鼠补体Ｃ３抗血清，为小鼠血清Ｃ３含量的测定提供试剂。利用菊糖非特异性吸附小鼠血清Ｃ３，制备兔抗小鼠补体Ｃ３抗血清免疫复合物，以此作为免疫原免疫健康雄兔，制得兔抗小鼠Ｃ３抗血清。用双向免疫扩散法测得抗血清效价为１∶１６～１∶３２；琼脂糖单向免疫扩散抗血清效价为１∶４０；与小鼠血清进行免疫电泳出现一条补体Ｃ３的沉淀线。
Rabbit anti-mouse complement C3 antiserum was prepared to provide reagents for the determination of serum C3 levels in mice. Rabbit anti-mouse C3 antiserum was prepared by non-specific inoculation of mouse serum C3 with inulin to prepare rabbit anti-mouse complement C3 antiserum immune complex, which was used as immunogen to immunize healthy male rabbits. The titers of antisera measured by two-dimensional immunodiffusion method were 1:16 ~ 1:32. The antiserum titer of one-way immunodiffusion was 1:40 with agarose, and a precipitation line of complement C3 was observed by immunoelectrophoresis with mouse serum.