Cell couplings before and after neural induction in embryos of Cynops orientalis were studied by means of single cell injection of Lucifer Yellow. Differences both in incidence and the extent of cell couplings were demonstrated. Results of cell couplings were correlated with electron microscopic observations of freeze-etching replicas. Cell couplings before and after neural induction in embryos of Cynops orientalis were studied by means of single cell injection of Lucifer Yellow. Differences both in incidence and the extent of cell couplings were demonstrated. Results of cell couplings were correlated with electron microscopic observations of freeze- etching replicas.