论文部分内容阅读
目的 了解乙型肝炎患者补体介导ＨＢｓＡｇ特异性免疫沉淀的抑制作用（ⅡＰＣ）低下在乙型肝炎发病中的作用。方法 采用 ＰＥＧ沉淀法测定患者血清循环免疫复合物（ＣＩＣ），以 ＨＢｓＡｇ和抗－ＨＢｓ制备特异性免疫复合物（ＩＣ），加入补体（血清）抑制其沉淀，并定量测定上清中的ＨＢｓＡｇ，确定ＩＩＰＣ功能，同时用单向琼脂扩散法测定Ｃ３、Ｃ４。结果 患者组ＣＩＣ检出阳性率为４５．０％～８２．６％，均显著高于正常组Ｐ＜０．０５～０．００１，正常组ⅡＰＣ后上清中ＨＢｓＡｇ浓度为 ０．４５±０．５８ｎｇ，急性肝炎、慢活肝、重症肝炎均显著降低（Ｐ＜ ０．００１），肝硬化组亦明显降低（ Ｐ＜ ０ ．０５），Ｃ３、Ｃ４在患者组亦有不同程度的降低。结论 乙型肝炎患者血清中存在特异性ＩＣ，患者ⅡＰＣ明显降低在乙型肝炎的发病中有一定作用，补体成分的减少与ⅡＰＣ的降低有一定关系。
Objective To investigate the role of complement-mediated inhibition of HBsAg-specific immunoprecipitation (ⅡPC) in the pathogenesis of hepatitis B in patients with hepatitis B infection. Methods Serum circulating immune complexes (CICs) were determined by PEG precipitation method. Specific immune complexes (ICs) were prepared by HBsAg and anti-HBs. Complement (serum) was added to inhibit its precipitation. Quantitative determination of HBsAg, Determine IIPC function, while using one-way agar diffusion method for the determination of C3, C4. Results The positive rate of CIC in patients was 45.0% ~ 82.6%, which were significantly higher than those in normal group (P <0.05 ~ 0.001). The concentration of HBsAg in supernatant of normal group ⅡPC was 0.45 ± 0 .58ng, acute hepatitis, slow-living liver and severe hepatitis were significantly decreased (P <0.001), and cirrhosis group was also significantly lower (P <0 .05), C3, C4 in the patient group also have different degrees of reduction. Conclusion There is a specific IC in the serum of patients with hepatitis B, and the significant reduction of Ⅱ PC in patients has a certain role in the pathogenesis of hepatitis B. The decrease of complement components is related to the reduction of Ⅱ PC.