【摘 要】
：
AIM: Peroxynitrite (ONOO-) is a powerful oxidant shown to damage membranes. In the present study, the effect of taurine on changes of liver plasma membrane Na+,
【机 构】
：
partment of Biochemistry
论文部分内容阅读
AIM: Peroxynitrite (ONOO-) is a powerful oxidant shown to damage membranes. In the present study, the effect of taurine on changes of liver plasma membrane Na+, K+-ATPase induced by ONOO- was investigated. METHODS: Liver plasma membrane was exposed toONOO-with or without taurine. Na+, K+-ATPase activity and lipid peroxidation as thiobarbituric acid reactive substances (TBARS) levels were measured.RESULTS: Different concentrations of ONOO- (100, 200,500, and 1 000 μmol/L) were found to decrease liver plasma membrane Na+, K+-ATPase activity significantly. The depletion of enzyme activity was not concentration dependent. Effects of different concentrations of taurine on liver plasma membrane Na+, K+-ATPase activity were also measured. Taurine did not cause any increase in enzyme activity. When plasma membranes were treated with 200 μmol/L ONOO- with different concentrations of taurine, a restoring effect of taurine on enzyme activity was observed. TBARS levels were also measured and taurine was found to decrease the elevated values. CONCLUSION: Taurine is observed to act as an antioxidant of ONOO-to decrease lipid peroxidation and thus affect liver plasma membrane Na+, K+-ATPase by restoring its activity.
其他文献
We describe here a case of 51-year-old woman with a symptomatic hepatic cyst that was misdiagnosed as a gastric submucosal tumor (SMT) with endoscopic ultrasoun
Using a wavelet function as the filter function of Fourier self-deconvolution, a new me- thod of resolving overlapped peaks, wavelet-Fourier self-deconvolution,
Primary stomach lymphoblastic B-cell lymphoma (B-LBL) is a rare tumor. We describe a primary stomach B-LBL in a 38 years old female who presented with nonspecif
echanisms of electron transfer of carbazole (CZ) and 9-ethylcarbazole (ECZ) were studied by electrochemistry and in-situ spectroelectrochemistry. The result ind
采用室内水培法，研究了2 mg·L-1五价砷胁迫下，外源添加不同浓度（0、10、20、40、60 mg·L-1）3-吲哚乙酸（IAA）14 d对砷超富集植物大叶井口边草（Pteris cretica var. nervosa）和同属的
Norfloxacin methanol solvate (1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinoline carboxylic acid methanol solvate) has been prepared. The crystal
A 1D chain-like organic-inorganic hybrid rare earth derivative of polyoxometalate (DMSO) and characterized by elemental analysis, IR spectra and single crystal
AIM: To study the implication of prokineticin 1 (PK1/EG VEGF) and prokineticin 2 (PK2/Bv8) in hepatocellular carcinoma angiogenesis. METHODS: The gene induction
针对如何提高氨法烟气脱硫装置脱硫效率的预测精度,建立了以烟气量、喷淋浆液密度、液气比、喷淋塔pH值、预洗涤塔pH值和浓缩槽pH值为输入变量,以脱硫效率为输出量的最小二乘
An 81-year-old man presented with epigastric pain and weight loss for one month. He had a past history of pulmonary tuberculosis, 10 years ago. We performed a g