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肿瘤坏死因子α(TNF_α)是由巨噬细胞分泌产生的细胞因子,具有免疫调节、抗肿瘤和细胞毒效应。随着对TNF_α生理、病理作者机制的深入研究,迫切需要建立一种既特异又敏感的TNF_α检测方法,用于测定体液,如血清和细胞培养上清中的TNF_α含量,由于TNF_β和TNF_α能结合相同的受体导致相应靶细胞裂解,因此常规的TNF_α生物活性检测方法缺乏特异性。许多已经建立的ELISA 检测法虽具有TNF_α特异性,但敏感性尚不如生物活性检测法。本文作者以纯化抗人TNF_α单克隆抗体(Sμg/ml)包被板,基因重组人TNF_α作标准抗原,利用
Tumor necrosis factor alpha (TNF alpha) is a cytokine secreted by macrophages and has immunomodulatory, anti-tumor and cytotoxic effects. With the in-depth study on the mechanism of TNF_α physiological and pathological mechanism, it is urgent to establish a specific and sensitive TNF_α detection method for the determination of TNFα content in body fluids, such as serum and cell culture supernatant, due to TNF_β and TNF_α binding The same receptor leads to lysis of the corresponding target cells, so the conventional method for detecting the biological activity of TNF-α lacks specificity. Although many established ELISA assays have TNF-alpha specificity, they are not as sensitive as bioassays. In this paper, the purified anti-human TNFα monoclonal antibody (Sμg / ml) coated plate, gene recombinant human TNF_α as a standard antigen, the use of