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目的为鱼腥草的生物捡定法研究提供合适的提取方法和菌株。方法鱼腥草提取液用正交实验提取,采用体外抑菌实验,用二倍稀释法,观察鱼腥草提取液对金黄色葡萄球菌、大肠埃希菌、枯草芽孢杆菌、产气肠杆菌的抑菌作用。结果鱼腥草生物检定法的最佳提取方法为:用40%的乙醇,80℃提取二次,第一次加10倍量溶剂,提取80min,第二次加8倍量溶剂,提取60min;枯草芽孢杆菌作为鱼腥草的生物检定法研究的标准菌株较为适宜。结论优选出的提取方法和标准菌株科学合理。
Objective To provide suitable methods and strains for the biological determination of Houttuynia cordata. Methods Houttuynia cordata extract was extracted by orthogonal experiment. Antibacterial activity was tested in vitro. The effect of Houttuynia cordata extract on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Enterobacter aerogenes Antibacterial effect. Results The best method of bioassay for Houttuynia cordata was as follows: extracted with 40% ethanol at 80 ℃ for twice, the first time with 10 times of solvent, extracted for 80 minutes, the second with 8 times of solvent, extracted for 60 minutes; Bacillus subtilis as Houttuynia biological test method for the standard strain is more appropriate. Conclusion The optimized extraction method and standard strain are scientific and reasonable.