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对盐、旱逆境下转BADH基因小麦品系99T6的生长发育、甜菜碱醛脱氢酶活性及甜菜碱积累等进行了研究分析,结果表明,转BADH基因株系在盐逆境下具有明显的生长优势,耐盐能力达到100mmol/L;电导率和质膜相对透性表明转基因株系抗膜损伤能力增强;甜菜碱醛脱氢酶活性的增强和甜菜碱积累的增加,说明转基因株系的盐旱逆境抗性是通过甜菜碱积累这一长期、持久的方式解除渗透胁迫,而不是通过脯氨酸积累这种临时的应急反应;以甜菜碱作为靶标性状采用基因工程方法提高植物盐旱耐性是可行的。
The growth and development of 99T6 BADH transgenic wheat line, the activity of betaine aldehyde dehydrogenase and the accumulation of betaine were studied under salt and drought stress. The results showed that the transgenic BADH lines had obvious growth advantages under salt stress , Salt tolerance of 100mmol / L; electrical conductivity and plasma membrane relative permeability of transgenic lines showed enhanced resistance to membrane damage; betaine aldehyde dehydrogenase activity and betaine accumulation increased, indicating that the transgenic lines of salt and drought Adversity resistance is a temporary, long-lasting way to relieve osmotic stress through the long-term, long-lasting accumulation of betaine, rather than through temporary accumulation of proline; it is feasible to use betaine as a target trait to increase salt tolerance in plants by genetic engineering of.