利用基因枪法将单个B .t.基因或与SBTi基因一起导入到两个华南地区优良籼稻品种中 ,获得 2 1个转B .t.单基因的植株系 ,4个转B .t.和SBTi双基因的植株系。对R1代植株的分子杂交和遗传分析表明 ,3个转双基因系中多个拷贝的B .t.和SBTi基因均是整合在植株基因组同一染色体上相同或相近位点。Northernblot证明在R2 代转基因植株中B .t .基因稳定表达。对稻纵卷叶螟的抗性实验表明 ,转B .t .单基因或B .t.和SBTi双基因的转基因植株均较原种对照有更强的抗性 ,而转双基因植株较转单基因植株又有更强的抗性。 A single B.t. gene was introduced into two elite indica rice varieties in southern China with the SBTi gene by particle bombardment to obtain 21 transgenic plants with Bt gene and 4 transgenic Bt and SBTi Double gene plant lines. The molecular hybridization and genetic analysis of the R1 plants showed that the Bt and SBTi genes of multiple copies in the three transgene lines were integrated into the same or similar sites on the same chromosome of the plant genome. Northern blot confirmed that the Bt gene is stable in R2 transgenic plants. The resistance to rice leaf roller Cnaphalocrocis medinalis experiments showed that transgenic Bt single gene or Bt and SBTi transgenic double transgenic plants are more resistant than the original control, Single-gene plants have stronger resistance.