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目的:研究低灌注状态脑组织神经肽Y1受体的mRNA表达。方法:建立左侧颈外静脉与颈总动脉端侧吻合的大鼠模型,测定吻合形成后脑血流动力学及脑组织神经肽Y(NPY)改变,检测该组织NPY1R mRNA表达。结果:大鼠静脉动脉端侧吻合形成动静脉分流,左侧局部脑血流量显著降低,脑组织中NPY增加,NPY1R mRNA表达下降。吻合口阻断后,左侧大脑血流量显著增加,NPY1R mRNA表达进一步减低。结论:长期低灌注脑缺血后NPY1R减少,使脑血管自动调节功能下降导致“正常灌注压突破”。
Objective: To study the mRNA expression of neuropeptide Y1 receptor in brain tissue under hypoperfusion. Methods: The left lateral jugular vein and common carotid artery end-to-side anastomosis rat model was established. The cerebral hemodynamics and neuropeptide Y (NPY) changes after anastomosis were measured. The expression of NPY1R mRNA in this tissue was detected. Results: Arteriovenous shunt was formed by the anastomosis of venous artery in rats, the left cerebral blood flow decreased significantly, NPY in brain tissue increased and NPY1R mRNA expression decreased. After anastomotic occlusion, left cerebral blood flow increased significantly, NPY1R mRNA expression was further reduced. CONCLUSION: The decrease of NPY1R after long-term hypoperfusion and cerebral vascular autoregulation results in “normal perfusion pressure breakthrough”.