论文部分内容阅读
目的:建立泻肝安神丸的定性及定量方法。方法:采用薄层色谱法对制剂中的当归、栀子、黄芩、远志进行定性鉴别;采用高效液相色谱法测定制剂中龙胆苦苷的含量。结果:薄层色谱显色清晰且阴性对照无干扰。龙胆苦苷在0.010 2~0.204 0 mg.mL-1浓度范围内呈良好的线性关系,平均回收率为98.2%,RSD=1.2%。结论:该法操作简便,结果准确,重复性好,可用于该制剂的质量控制。
Objective: To establish qualitative and quantitative method of Xiegan Anshen pill. Methods: The Angelica, Fructus Gardeniae, Scutellaria baicalensis and Polygala tenuifolia were identified by TLC. The content of gentiopicroside in the preparation was determined by HPLC. Results: Thin-layer chromatography developed clear and negative controls without interference. Gentiopicrin showed a good linear relationship in the concentration range of 0.010 2 ~ 0.204 0 mg.mL-1 with the average recovery of 98.2% and RSD = 1.2%. Conclusion: The method is simple, accurate, reproducible and can be used for the quality control of the preparation.