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通过人工合成的人类免疫缺陷症病毒(Humanimmunodeficiencyvirus,HIV)糖蛋白肽GP120对人和无脊椎动物(Mytilusedulis)免疫细胞的抑制作用的研究。人单核细胞和Mytilusedulis免疫细胞分别与GP120保温后,均抑制细胞的吞噬细菌(Psudomonasstretzi)作用。应用计算机显微图像术(Computer-assistedmicroscopy)直接计算细菌被吞噬的情况,并测定细胞形状改变的参数。结果发现,细菌存在时,GP120使免疫细胞活性降低,细胞变圆(形状因子=0.86±0.06), 而对照组免疫细胞则因细菌的刺激作用变长或阿米巴样(形状因子<0.5),噬菌能力明显增加,提示GP120是AIDS病人细胞免疫机制受损害的原因之一。
Inhibition of Human and Invertebrate (Mytilusedulis) Immune Cells by a Synthetic Humanimmunodeficiencyvirus (HIV) Glycoprotein GP120. Human monocytes and Mytilusedulis immune cells, respectively, incubated with GP120, inhibited the cell’s phagocyte (Psudomonasstretzi) effect. Computer-assisted microscopy was used to directly calculate the phagocytosis of bacteria and to determine the parameters of the shape change of the cells. The results showed that in the presence of bacteria, GP120 decreased immune cell activity, rounded cells (shape factor = 0.86 ± 0.06), while immune cells in the control group became longer due to bacterial stimulation or amoebic (shape Factor <0.5), phagocytosis increased significantly, suggesting that GP120 is one of the causes of cellular immune damage in AIDS patients.