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腺病毒载体是近年来继逆转录病毒载体之后被广泛应用的一个基因运载系统。它具有宿主细胞广泛、繁殖滴度高、不整合及相对安全等特点,在基因治疗中发挥越来越大的作用。本文介绍了以E1区缺失为特征的第一代腺病毒载体应用的有效性、安全性等方面的研究情况,和旨在克服第一代腺病毒载体不足的第二代载体构建方面的进展。
Adenovirus vector is a gene delivery system widely used in recent years after retroviral vector. It has a wide range of host cells, breeding high titer, integration and relative safety features, play an increasingly important role in gene therapy. This article introduces the research status of the first generation adenovirus vectors characterized by the deletion of E1 region and the progress in the construction of the second generation vectors aimed at overcoming the deficiency of the first generation adenoviral vectors.