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为探索卵巢局部瘦素信号缺失对卵巢自身功能的影响,进行以下研究:①对瘦素长受体(LR)缺失(LR-)的雌性B6.Cg-m+/+Leprdb小鼠及其同窝出生的有完整LR的雌性野生型C57BL/6J小鼠(LR+)进行卵巢交互移植,在同一雌性野生型C57BL/6J小鼠体内构建两侧卵巢不同的LR基因型,分别标记为KO(卵巢LR-)、WT(卵巢LR+)两组。②监测卵巢的周期变化;动情周期恢复后各组均予GnRH-a降调节后取材,RT-PCR检测分子生物学指标。结果显示:①卵巢移植术后小鼠仍有正常的动情周期,经GnRH-a降调节后,小鼠失去动情周期的变化;②RT-PCR结果显示,KO组卵巢中Star、Cyp17、Cyp19、Jak2、Stat3、Pias3mR-NA的表达量均明显低于WT组,Ob-RbmRNA在KO组卵巢中无表达,WT组有表达,Socs3mRNA在两组间表达无明显差异。研究表明,卵巢内存在完整的瘦素信号传导通路;瘦素受体缺失及外周糖脂代谢环境对卵巢功能的影响并不重要,重要的是下丘脑-垂体对卵巢的调控作用,其对卵巢作用的分子机制是调控卵巢甾体激素合成酶基因的表达。
To investigate the effect of local ovarian leptin deficiency on the ovarian function, the following studies were carried out: (1) Female B6.Cg-m + / + Leprdb mice lacking leptin long receptor (LR) and their littermates Transgenic ovariectomized female C57BL / 6J mice (LR +) with intact LR were born. Different LR genotypes of ovaries were constructed in the same female wild type C57BL / 6J mice and labeled as KO (ovary LR -), WT (ovarian LR +) two groups. ② monitoring of ovarian cycle changes; recovery of estrous cycle after each group were GnRH-a down regulation of the material, RT-PCR detection of molecular biological indicators. The results showed that: (1) Mice still had normal estrous cycle after ovarian transplantation, and the mice lost the estrous cycle after GnRH-a down regulation. (2) The results of RT-PCR showed that the expression of Star, Cyp17, Cyp19, Jak2 , Stat3, Pias3mR-NA were significantly lower in WT group than those in WT group. There was no expression of Ob-Rb mRNA in ovary of WT group and WT group. The expression of Socs3 mRNA was not significantly different between the two groups. Studies have shown that there is a complete ovarian leptin signaling pathway; lack of leptin receptor and peripheral glucose and lipid metabolism of the environment on the ovarian function is not important, important is the hypothalamus - pituitary regulation of the ovary, the ovary The molecular mechanism of action is the regulation of ovarian steroid hormone synthase gene expression.