论文部分内容阅读
吗氯贝胺系新型可逆性。选择性单胺氧化酶Ａ型抑制剂。本文首次在国内建立测定人血清中吗氯贝胺的ＨＰＬＣ法，并报告部分病例血清标本的测定结果。以甲氧氯普胺作内标，在碱性条件下用乙醚提取。流动相为乙腈：０．０２３ｍｏ１／Ｌ四甲基乙二胺溶液（２０：８０，Ｖ／Ｖ，ｐＨ６．０）。用紫外２４０ｎｍ测定。吗氯贝胺线性范围为０－４０００μｇ／Ｌ，最低检出限４μｇ／Ｌ。吗氯贝胺的绝对回收率大于９０％，日内变异为１．６－３．２％，日间变异为２．４－５．３％。应用本法对服用吗氯贝胺治疗的１８例抑郁症病人的５４份血清标本进行测定，结果表明本方法与国外同类方法具有可比性。
Moclobemide series of new reversible. Selective monoamine oxidase type A inhibitors. This article for the first time in China to establish a HPLC method for the determination of moclobemide in human serum and report the results of the serum samples in some cases. Metoclopramide as an internal standard, in ether under ether extraction conditions. The mobile phase was acetonitrile: 0.023 mol / L tetramethylethylenediamine solution (20:80, V / V, pH 6.0). Measured with UV 240nm. Moclobemide linear range of 0-4000μg / L, the minimum detection limit of 4μg / L. The absolute recovery of moclobemide was greater than 90% with an intraday variation of 1.6-3.2% and an intraday variation of 2.4-5.3%. This method was applied to the determination of 54 serum samples of 18 patients with depression treated with clomipine, and the results showed that the method was comparable with that of similar foreign methods.