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本文研究薄皮甜瓜脂氧合酶(lipoxygenase,LOXs)及其基因家族成员(Cm LOXs)在接种枯萎病后的响应。以薄皮甜瓜‘玉美人’幼苗为试材,“三叶一心”时,灌根法接种枯萎病菌-尖孢镰刀菌(Fusarium oxysporum f.sp.melonis),在接种后不同时间测定了幼苗电解质渗透率、根系活力、LOX活性及Cm LOXs基因相对表达量。接种枯萎病菌后甜瓜叶片中有13个CmLOXs基因在接种枯萎病菌后上调表达。其中除Cm LOX02和Cm LOX11在第3天显著升高外,其他11个基因(Cm LOX03、CmLOX07~10、Cm LOX12~17)均在第5天时相对表达量达到最大值。甜瓜根部有5个Cm LOXs基因上调表达,其中Cm LOX03、Cm LOX16和Cm LOX17在处理1 d后有显著升高,Cm LOX05和Cm LOX06在接种3 d时与对照有显著差异。薄皮甜瓜幼苗在接种枯萎病菌时,根系活力显著下降,电解质渗透率升高,叶片和根中LOX活性升高,叶片和根中分别有13个和5个Cm LOXs成员上调表达,但响应时间不同,这些结果表明Cm LOXs可能参与防御反应,本研究为后续通过转基因技术研究其在生物胁迫中的作用奠定基础。
In this paper, we investigated the response of lipoxygenase (LOXs) and their gene members (Cm LOXs) to Fusarium wilt. The seedlings of Fusarium oxysporum f.sp. melonis were inoculated with Fusarium oxysporum f.sp. melonis at the time of inoculation, Electrolyte permeability, root activity, LOX activity and Cm LOXs gene relative expression. After inoculation with Fusarium wilt, 13 CmLOXs genes in melon leaves were up-regulated after Fusarium wilt inoculation. In addition to Cm LOX02 and Cm LOX11 significantly increased on the 3rd day, the other 11 genes (Cm LOX03, CmLOX07 ~ 10, Cm LOX12 ~ 17) on the 5th day when the relative expression reached the maximum value. Five Cm LOXs genes were up-regulated in the roots of melon, in which Cm LOX03, Cm LOX16 and Cm LOX17 were significantly increased after 1 day of treatment, while Cm LOX05 and Cm LOX06 were significantly different from the control at 3 days after inoculation. When inoculated with Fusarium oxysporum, the root vitality decreased significantly, the electrolyte permeability increased, the LOX activity in leaves and roots increased, and 13 and 5 Cm LOXs in leaves and roots were up-regulated respectively, but the response time was different . These results indicate that Cm LOXs may be involved in the defense response. This study laid the foundation for the subsequent study of its role in biotic stress through transgenic technology.