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为明确海带多糖抗病毒剂对烟草抗烟草花叶病毒(Tobacco mosaic virus,TMV)病的诱导抗性及其对TMV感染后的保护作用,采用间接酶联免疫吸附法(ELISA)研究0.5 mg/mL海带多糖水剂、0.5 mg/mL香菇多糖水剂、6.25 mg/L氨基寡糖素水剂和0.1 mg/mL盐酸吗啉胍.铜可湿性粉剂对TMV侵染的预防保护作用和对TMV感染植株的治疗作用,并检测施用海带多糖后烟草体内过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)及超氧化物歧化酶(SOD)活性的变化和海带多糖对感染TMV后烟草叶绿素含量的影响。接种前喷施0.5 mg/mL海带多糖后,再接种TMV病毒,其抑制率可达42.42%,与喷施6.25 mg/L氨基寡糖素抑制率44.96%的效果相当,预防保护作用较好;但两种药剂处理感病植株的治疗效果较差,其抑制率仅分别为38.93%和40.13%。海带多糖能够系统地诱导烟草体内POD、PAL及SOD活性,从而控制了感染TMV后烟草叶绿素含量下降。说明海带多糖可诱导植物产生抗病性。
In order to clarify the induction resistance of Tobacco mosaic virus (Tobacco mosaic virus) and its protective effect on TMV after infection by TMV, indirect enzyme-linked immunosorbent assay (ELISA) was used to study the effect of 0.5 mg / mL seaweed polysaccharide, 0.5 mg / mL lentinan, 6.25 mg / L aminogalactan and 0.1 mg / mL morpholine guanidine.Prevention and protection of copper WP on TMV infection and inhibition of TMV (POD), phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activities in the tobacco and the effect of kelp polysaccharide on the infection of TMV Effects of post-tobacco chlorophyll content. Pretreatment with 0.5 mg / mL kelp polysaccharide, and then inoculated with TMV virus, the inhibition rate up to 42.42%, spraying 6.25 mg / L aminoglycoside inhibition rate of 44.96% of the effect, prevention and protection is better; However, the curative effect of the two agents on susceptible plants was poor with the inhibition rates of 38.93% and 40.13% respectively. Laminaria polysaccharides could systematically induce POD, PAL and SOD activities in tobacco and control the decrease of chlorophyll content in tobacco after TMV infection. It shows that kelp polysaccharides can induce plants to produce disease resistance.