用HPLC法测定复方利福平片中利福平、异烟肼及吡嗪酰胺的含量。方法 :采用SpherisorbCN(2 50mm× 4 6mm ,5μm)色谱柱 ,流动相采用 0 0 1mol/L庚烷磺酸钠溶液 (pH2 2 )—乙腈 (44∶56,v/v) ,流速为 2 0ml/min,检测波长为 2 54nm。结果 :利福平在 71 76～ 1 66 40 μg/ml、异烟肼在 47 64～ 1 1 1 1 6μg/ml、吡嗪酰胺在 1 37 76～ 32 1 44μg/ml范围内线性良好 ,r分别为 0 9999、 0 9991、 0 9999(n =9) ,平均回收率分别为 :99 57%、 99 51 %、 1 0 0 1 0 % ,RSD分别为 :0 71 %、 0 51 %、0 62 % (n =7)。结论 :本法简便、准确、灵敏度高 Determination of rifampicin, isoniazid and pyrazinamide in compound rifampicin tablets by HPLC method. METHODS: SpherisorbCN (2 50mm × 4 6mm, 5μm) column was used. The mobile phase consisted of 0 0 1mol / L sodium heptane sulfonate solution (pH2 2) -acetonitrile (44:56, v / v) / min, detection wavelength of 2 54nm. Results: Rifampicin was linear at 71 76-1 66 40 μg / ml and isoniazid at 47 64-1 1 1 1 6μg / ml. Pyrazinamide showed a good linearity over the range of 1 37 76- 32 1 44 μg / ml. R Respectively. The average recoveries were 99 57%, 99 51% and 100 0% respectively. The RSDs were 0 71%, 0 51%, 0 0 62% (n = 7). Conclusion: This method is simple, accurate and sensitive