论文部分内容阅读
研究用原子吸收光谱法(AAS)测定中药黄芩水煎液中Cu,Zn,Fe和Mn化学形态的分析方法。水煎液经0.45μm滤膜过滤后分为悬浮态和可溶态组分试样;对于可溶态组分试样,采用LSA-10大孔树脂将其分为有机态和无机态组分试样,并采用正辛醇-水分配体系,在模拟人体胃肠环境条件下,将水可溶态中待测元素分离为醇溶态和水溶态,然后用AAS测定上述各组分试样中待测元素的含量,即可给出比现有的仅测量中药黄芩中上述各待测元素总量为多的信息。该法待测元素Cu,Zn,Mn的检出限(3倍空白标准差)为0.01μg.mL-1,Fe为0.02μg.mL-1,精密度(RSD,n=11)为1.5%～3.6%,对可溶态和无机态组分试样进行加标测定的回收率为96.7%～105.0%。所得结果对黄芩的综合研究有重要的实际意义。
To study the determination of chemical forms of Cu, Zn, Fe and Mn in Scutellaria baicalensis decoction by atomic absorption spectrometry (AAS). The decoction was filtered through 0.45μm membrane and divided into suspended and soluble components of the sample; for the soluble components of the sample, using LSA-10 macroporous resin is divided into organic and inorganic components Octanol-water partitioning system was used to separate the water-soluble elements to be tested in the alcohol-soluble and water-soluble state under simulated gastrointestinal conditions. Then, the above-mentioned components were measured by AAS In the content of the elements to be tested, to give more than the existing only to measure the Chinese medicine Scutellaria total amount of the above elements to be tested for more information. The detection limit (3 times of blank standard deviation) of Cu, Zn and Mn in this method was 0.01μg.mL-1, Fe was 0.02μg.mL-1, and the precision (RSD, n = 11) was 1.5% ~ 3.6%. The spiked recoveries of soluble and inorganic components were 96.7% ~ 105.0%. The results obtained on the comprehensive study of Scutellaria has important practical significance.