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目的探讨叶黄素(lutein)对异丙肾上腺素(ISO)诱导的大鼠心肌纤维化保护作用及机制。方法 60只大鼠随机分为6组,除对照组外,其余各组大鼠皮下注射ISO 5 mg/kg,连续7 d;造模第2 d,低、中、高剂量叶黄素组大鼠灌胃给予lutein 20、40、80 mg/kg,阳性对照组大鼠给予卡托普利(cap)10 mg/kg,模型组和对照组大鼠给予等量蒸馏水,连续28 d。检测大鼠心脏指数、心电图、运动耐力、心肌组织中羟脯氨酸(HYP)、谷胱甘肽(GSH)、丙二醛(MDA)含量和血清中超氧化物歧化酶(SOD)含量;苏木素-伊红染色(HE)观察大鼠心肌病理学变化。结果与对照组比较,模型组大鼠运动耐力降低、心指数与左心室指数[分别为(3.73±0.58)、(2.57±0.43)mg/g]增加(P<0.01),心率明显增加,心肌组织中HYP、MDA含量[分别为(0.48±0.07)g/mg、(2.37±0.84)nmol/mgpr]升高(P<0.05),心肌组织中GSH含量、血清中SOD水平[分别为(55.19±2.08)U/mg、(512.17±26.48)U/m L]明显下降(P<0.01),心肌组织间质成分增多,细胞核间距较宽,有大量炎性细胞浸润且纤维组织增生伴心肌萎缩;与模型组比较,高剂量叶黄素组大鼠运动耐力提高、心指数与左心室指数[分别为(3.02±0.28)、(2.09±0.17)mg/g]降低(P<0.01),心肌组织中HYP和丙二醛含量[分别为(0.28±0.06)g/mg、(1.89±0.61)nmol/mgpr]明显降低(P<0.05),心肌组织中GSH含量、血清中SOD水平[分别为(72.70±0.05)U/mg、(571.19±15.58)U/m L]明显升高(P<0.01),心肌组织间质成分略增多,无炎细胞浸润,可见轻度纤维组织增生,无心肌萎缩。结论叶黄素对异丙肾上腺素所致大鼠心肌纤维化具有一定保护作用,其机制可能与改善大鼠机体抗氧化能力有关。
Objective To investigate the protective effect of lutein on isoproterenol (ISO) -induced myocardial fibrosis in rats and its mechanism. Methods Sixty rats were randomly divided into 6 groups. Except for the control group, rats in each group were subcutaneously injected with ISO at a dose of 5 mg / kg for 7 days. On the 2nd day after modeling, the lutein group Rats were given intragastric administration of lutein 20,40,80 mg / kg, positive control group rats were given captopril 10 mg / kg, the model group and the control group were given equal volume of distilled water for 28 days. Cardiac index, electrocardiogram (ECG), exercise tolerance, hydroxyproline (HYP), glutathione (GSH) and malondialdehyde (MDA) Pathological changes of myocardium in rats were observed by eosin staining (HE). Results Compared with the control group, the exercise tolerance of the model group decreased, the heart index and the left ventricular index increased by (3.73 ± 0.58) and (2.57 ± 0.43) mg / g, respectively (P <0.01) The levels of HYP and MDA in myocardium [(0.48 ± 0.07) g / mg and (2.37 ± 0.84) nmol / mgpr], respectively ± 2.08) U / mg, (512.17 ± 26.48) U / m L] significantly decreased (P <0.01), myocardial interstitial components increased, the nucleus was wide, a large number of inflammatory cell infiltration and fibrous tissue hyperplasia with myocardial atrophy Compared with the model group, exercise tolerance of high dose lutein group increased, heart index and left ventricular index (3.02 ± 0.28, (2.09 ± 0.17) mg / g] decreased (P <0.01) The content of HYP and malondialdehyde in the tissue [(0.28 ± 0.06) g / mg and (1.89 ± 0.61) nmol / mgpr] were significantly lower than those in the control group (72.70 ± 0.05) U / mg, (571.19 ± 15.58) U / m L] (P <0.01). The interstitial components in myocardium slightly increased, with no infiltration of inflammatory cells, showing mild fibrous tissue hyperplasia without myocardium Shrinkage. Conclusion Lutein may protect isoproterenol-induced myocardial fibrosis in rats, and its mechanism may be related to the improvement of antioxidant capacity in rats.