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目的:探讨IgE介导综合征与HLA-DRB基因多态性的相关性,研究HLA-DRB等位基因与血清总IgE(TIgE)间的关系。方法:用引物序列特异性聚合酶链反应(PCR-SSCP)扩增HLA-DRB等位基因,用酶联免疫吸附实验(ELISA)检测血清TIgE,用皮肤过敏实验进行皮肤过敏原检测。结果:HLA-DRB等位基因在男女频率分布无显著性差异(P>0.05);DR3基因频率患者组为13.41％,对照组为3.75％(P<0.01,RR4.52)。而DR6基因频率在患者组为0,对照组为8.75％(P<0.01)。DR1等位基因与TIgE之间有显著性差异(P<0.05),且与低浓度有关。28例皮肤过敏原检测结果显示尘螨的检出率最高。结论:推测DR3基因位点是天津地区汉族IgE介导综合征的易感基因,DR6基因位点是天津地区汉族IgE介导综合征的抗性基因。HLA-DR1基因与血清TIgE有关。尘螨可能是诱发和加重该病的变应原。
Objective: To investigate the association between IgE-mediated syndrome and HLA-DRB gene polymorphisms and to study the relationship between HLA-DRB alleles and serum total IgE (TIgE). Methods: HLA-DRB alleles were amplified by PCR-SSCP, serum TIgE was detected by enzyme-linked immunosorbent assay (ELISA) and skin allergen was detected by skin allergy test. Results: There was no significant difference in the frequency distribution of HLA-DRB alleles between men and women (P> 0.05). The frequency of DR3 gene was 13.41% in patients and 3.75% in controls (P <0.01, RR4.52). The DR6 gene frequency was 0 in the patient group and 8.75% in the control group (P <0.01). There was a significant difference between DR1 allele and TIgE (P <0.05), which was related to low concentration. 28 cases of skin allergen test showed the highest detection rate of dust mites. Conclusion: It is speculated that DR3 locus is a predisposing gene of IgE-mediated syndrome in Han nationality in Tianjin. DR6 locus is a resistance gene of IgE-mediated syndrome in Han population of Tianjin. HLA-DR1 gene and serum TIgE related. Dust mites may be allergens that induce and exacerbate the disease.