论文部分内容阅读
目的研究慢性砷暴露对雌性大鼠的内分泌干扰作用。方法 50只4周龄雌性Wistar大鼠随机分成对照组(饮用去离子水)及砷暴露组(饮用砷含量分别为0.05、0.1、0.2和0.4μg/ml水),32周后采用放射免疫法测定血清雌二醇(E2)、孕酮(P)、黄体生成素(LH)、卵泡刺激素(FSH)、促性腺激素释放激素(GnRH)、垂体泌乳素(PRL)和皮质醇(Cort)含量。结果与对照组相比,各浓度砷暴露组E2和P含量均有升高趋势,LH水平有降低趋势,但差异均无显著性(P>0.05);FSH和PRL水平在低砷暴露组有升高趋势,而高砷暴露则有降低趋势(P>0.05);GnRH和Cort含量升高,且0.4μg/ml暴露组大鼠GnRH明显升高(P<0.05),0.1、0.4μg/ml组大鼠Cort升高明显(P<0.05)。结论慢性砷暴露可干扰雌性大鼠体内上述激素的水平。
Objective To study the endocrine disrupting effect of chronic arsenic exposure on female rats. Methods Fifty four-week-old female Wistar rats were randomly divided into control group (drinking deionized water) and arsenic exposure group (drinking arsenic levels of 0.05, 0.1, 0.2 and 0.4 μg / ml, respectively) Serum levels of estradiol, progesterone, LH, FSH, GnRH, PRL and Cort were measured. content. Results Compared with the control group, the concentrations of E2 and P in the arsenic exposure groups increased and the levels of LH decreased, but the differences were not significant (P> 0.05). The levels of FSH and PRL in the low arsenic exposure group (P> 0.05). The contents of GnRH and Cort increased, and the GnRH of 0.4μg / ml exposure group was significantly increased (P <0.05), while 0.1,0.4μg / ml Rats in group Cort increased significantly (P <0.05). Conclusion Chronic arsenic exposure may interfere with the levels of these hormones in female rats.