目的筛选出与疾病相关的蛋白质分子。方法采用龈沟冲洗法获取龈沟液，用微量自动快速电泳仪分析了８６例龈沟液样本（其中健康龈２０例、龈炎２４例、牙周炎４２例）。十二烷基磺酸钠－聚丙烯酰胺电泳分离的蛋白质区带用快速图象分析仪进行测量。结果在炎症情况下分子量为８２０００、７７０００及１７０００的血清源蛋白质发生率较健康龈升高，分子量为４１０００、２１０００、１２０００的非血清源蛋白质发生率亦较健康龈高，丰度值大。结论分子量为８２０００、４１０００、２１０００及１２０００的蛋白质可能与牙周炎有较密切的关系。 Objective To screen protein molecules related to diseases. Methods Gingival crevicular fluid was obtained by gingival crevicular flushing method. 86 gingival crevicular fluid samples (including 20 healthy gingiva, 24 gingivitis and 42 periodontitis) were analyzed by micro automatic rapid electrophoresis. Protein bands separated by sodium dodecyl sulfate-polyacrylamide electrophoresis were measured with a rapid image analyzer. Results The incidence of serum protein with 82,000, 77,000 and 17,000 molecular weight was higher than that of healthy gingiva under inflammatory conditions. The incidence of non-serum protein with molecular weight of 41,000, 21,000 and 12,000 was also higher than that of healthy gingival and the abundance was high. Conclusion Proteins with molecular weights of 82000, 41000, 21000 and 12000 may have a close relationship with periodontitis.