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目的:建立同时测定珍珠菜中芦丁和柚皮素-7-O-葡萄糖苷含量的高效液相色谱方法。方法:以Diamonsil C18柱(4.6mm×250mm,5μm)为色谱柱,流动相采用乙腈(A)-0.1%磷酸(B)梯度洗脱:0～18min,83%～80%B;18～30min,80%～86%B。芦丁和柚皮素-7-O-葡萄糖苷的检测波长分别为254nm和281nm,流速为1.0mL/min,柱温为35℃,进样量为20μL。结果:用梯度洗脱得到了较好的分离效果,芦丁在1.00～48.00μg/mL之间线性关系良好,r=0.9993;柚皮素-7-O-葡萄糖苷在0.64～40.72μg/mL之间线性关系良好,r=0.9998。结论:该方法准确,简便,可用于珍珠菜中芦丁和柚皮素-7-O-葡萄糖苷含量的同时测定。
Objective: To establish a HPLC method for the simultaneous determination of rutin and naringenin -7-O-glucoside in pearl dishes. Methods: Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) was used as the column. The mobile phase was eluted with a gradient of acetonitrile (A) -0.1% phosphoric acid (B): 0-18 min, 83-80% B; , 80% ~ 86% B. The detection wavelength of rutin and naringenin-7-O-glucoside was 254 nm and 281 nm respectively, the flow rate was 1.0 mL / min, the column temperature was 35 ℃ and the injection volume was 20 μL. Results: A good separation effect was obtained by gradient elution. Rutin had a good linearity between 1.00 ~ 48.00μg / mL, r = 0.9993. The concentration of naringenin -7-O-glucoside was in the range of 0.64 ~ 40.72μg / mL The linear relationship between good, r = 0.9998. Conclusion: The method is accurate and simple and can be used for the simultaneous determination of rutin and naringenin-7-O-glucoside in pearl.