论文部分内容阅读
目的为研究前列腺细菌感染和慢性前列腺炎之间潜在的关联,本文报告了应用实时PCR方法检测前列腺液(EPS)或按摩前列腺后的尿液(VB3)中的细菌。方法细菌模板DNA取自慢性前列腺炎患者的EPS或VB_3标本。1对通用引物:用于扩增所有已知细菌的核糖体DNA(16SrDNA)。3个荧光探针:一个为检测所有细菌的通用探针(Uniprobe);1个为检测大肠杆菌的特异性探针(Ecprobe);1个为检测金黄色葡萄球菌的特异性探针(SAprobe)。结果应用实时PCR方法检测慢性前列腺炎患者,80个标本中有28个检测到细菌,包括14个为大肠杆菌阳性,6个为金黄色葡萄球菌阳性。结论实时PCR具有能定量、快速、特异等优点,有望成为能对慢性前列腺炎患者EPS或VB_3中的细菌进行定性、定量检测的一种很有前途的方法。
Objective To investigate the potential association between BPH and chronic prostatitis, this paper reports the detection of bacteria in the prostate (EPS) or urine (VB3) after prostate massage using real-time PCR. Methods Bacterial template DNA was extracted from EPS or VB 3 specimens from patients with chronic prostatitis. 1 pair of universal primers: used to amplify ribosomal DNA (16S rDNA) of all known bacteria. Three fluorescent probes: one Uniprobe for detecting all bacteria, one Ecprobe for detection of Escherichia coli, one SAprobe for detection of Staphylococcus aureus, . RESULTS: Real-time PCR was used to detect chronic prostatitis in 28 of 80 samples, including 14 positive for Escherichia coli and 6 for Staphylococcus aureus. Conclusion Real-time PCR has the advantages of being quantitative, rapid and specific. It is expected to become a promising method for the qualitative and quantitative detection of bacteria in EPS or VB_3 of patients with chronic prostatitis.