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目的:探讨温补肾阳方对抑郁模型大鼠行为学及脑内单胺递质的影响。方法:选择体重和旷野试验(open-field test)评分相近的72只SD大鼠,随机分为空白组,模型组,温补肾阳方低、中、高剂量组,逍遥散大剂量组,应用分养和长期不可预知的中等强度应激造成大鼠抑郁模型,定期测定各组大鼠行为学,采用酶联接免疫吸附剂(ELISA)法检测大鼠5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)含量,观察温补肾阳方对该模型大鼠的疗效,并与较为公认可改善抑郁症的逍遥散进行疗效对比。结果:抑郁模型组大鼠Open-Field评分、糖水摄取量、5-HT、DA、NE明显低于空白组(P<0.05或P<0.01);各治疗组可改善大鼠Open-Field评分、糖水摄取量、5-HT、DA、NE,与模型组相比有统计学差异(P<0.05),各治疗组比较未见显著性差异。结论:温补肾阳方改善抑郁大鼠的抑郁状态与逍遥散相近,提示临床可采用温补肾阳法治疗抑郁症。
Objective: To investigate the effects of Warming Kidney Yang Formula on behavior and monoamine neurotransmitters in the depression model rats. Methods: Seventy-two SD rats with similar body weight and open-field test scores were randomly divided into blank group, model group, low, middle and high dose of warming yang and kidney yang, Xiaoyaosan high dose group, The rats model of depression was established by feeding and long-term unpredictable moderate-intensity stress. The behavior of rats in each group was measured regularly. The levels of 5-hydroxytryptamine (5-HT), dopamine DA) and norepinephrine (NE) were measured. The curative effect of Wuby Shenyang prescription on this model rat was observed and compared with that of Xiaoyao San, which is generally accepted to improve depression. Results: Open-Field score, water intake, 5-HT, DA and NE in depression model group were significantly lower than those in blank group (P <0.05 or P <0.01); Open-Field score, The intake of sugar, 5-HT, DA and NE had statistical difference compared with the model group (P <0.05), and there was no significant difference between the treatment groups. Conclusion: Wubyhenyangyang decoction can improve the depressive state of depression in rats and Xiaoyao San is similar, which suggests that we can treat the depression with the method of warming the kidney and invigorating the yang.