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目的了解湖南省食源性德尔卑沙门菌的耐药谱及分子分型,建立沙门菌指纹图谱数据库。方法运用肉汤稀释法对食源性德尔卑沙门菌进行药敏试验,并采用脉冲场凝胶电泳法(PFGE)进行分子分型。结果 47株德尔卑沙门菌中,有46株对1种以上抗生素耐药(97.87%),有29株多重耐药菌株(61.70%);德尔卑沙门菌分为32种PFGE型,其中有10种PFGE型的菌株数超过1株。结论湖南省食源性德尔卑沙门菌分离株呈高耐药和多重耐药趋势,PFGE分子型别呈多态性,有同源菌株存在,PFGE分型为食源性疾病溯源和预警提供技术基础。
Objective To understand the drug resistance spectrum and molecular typing of foodborne Salmonella delphi in Hunan Province and establish a database of Salmonella fingerprinting. Methods The drug-sensitivity test of food-borne Salmonella delphi was carried out by broth dilution method and the molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Results Among the 47 Salmonella delphi strains, 46 strains were resistant to more than one antibiotic (97.87%) and 29 were multi-resistant (61.70%). Salmonella delphi was divided into 32 PFGE types, of which 10 The number of PFGE-type strains exceeds 1 strain. Conclusion The food-borne Salmonella delphi in Hunan Province is highly resistant and multidrug-resistant. The genotypes of PFGE are polymorphic, and the presence of homologous strains. The PFGE genotyping provides a source for the tracing and early warning of food-borne diseases basis.